2482. ANA. Double-stranded DNA (dsDNA), IgG antibodies

The presence of antibodies to double-stranded (dsDNA) DNA is highly specific for systemic lupus erythematosus (SLE); they are less commonly detected and at lower concentrations in other diffuse connective tissue diseases or drug-induced SLE. IgG anti-dsDNA antibodies are presumed to be directly involved in the pathogenesis of vasculitis and lupus nephritis.

The diagnostic specificity of the IgG anti-dsDNA test for SLE (the percentage of negative test results in the absence of the disease) is 98% in a healthy donor population and 87% in a population of patients with other autoimmune diseases.

The diagnostic sensitivity of the test for SLE (the percentage of positive test results in the presence of the disease) is 85%.

The level of anti-dsDNA antibodies in patients with SLE directly correlates with the concentration of IgG circulating immune complexes. They are present at increased concentrations in the renal glomeruli of patients with SLE who have severe renal pathology.

dsDNA can bind to the glomerular basement membrane, which may lead to the formation of immune complexes directly within the glomeruli. The accumulation of immune complexes results in complement activation (with consumption of serum complement reserves) and the development of inflammation and tissue damage.

For the diagnosis, assessment of disease activity, and monitoring of treatment in systemic lupus erythematosus.

• Venous blood

In order to exclude factors that may affect the test results, we recommend to follow the preparation rules: