3161. PCR. Helicobacter pylori (stool, qualitative determination)

H. pylori is detected in most cases of gastritis and peptic ulcers and, in addition, greatly increases the risk of developing adenocarcinoma and B-cell lymphoma of the stomach. Modern diagnosis of these diseases is impossible without accurate and rapid identification of H. pylori.

H. pylori can be detected using several methods. Real-time polymerase chain reaction (RT-PCR) can detect DNA fragments of the infectious agent in stool. Real-time PCR has a high sensitivity (93-95%), which makes it possible to detect both helical and coked (uncultivated) forms of H. pylori.

To diagnose diseases caused by H. pylori: 

• atrophic or antral gastritis;
  
• stomach and duodenal ulcers.
  

A false negative result can result from: 

• presence of blood, bile, excess inorganic salts and other components in the feces sample;
  
• treatment with tetracycline, penicillin (aminopenicillins), macrolides (clarithromycin, azithromycin) and metronidazole.
  

• Stool

MATERIAL COLLECTION RULES