3161. PCR. Helicobacter pylori (stool, qualitative determination)
Description
H. pylori is detected in most cases of gastritis and peptic ulcers and, in addition, greatly increases the risk of developing adenocarcinoma and B-cell lymphoma of the stomach. Modern diagnosis of these diseases is impossible without accurate and rapid identification of H. pylori.
H. pylori can be detected using several methods. Real-time polymerase chain reaction (RT-PCR) can detect DNA fragments of the infectious agent in stool. Real-time PCR has a high sensitivity (93-95%), which makes it possible to detect both helical and coked (uncultivated) forms of H. pylori.
When and who needs the test?
To diagnose diseases caused by H. pylori:
- atrophic or antral gastritis;
- stomach and duodenal ulcers.
A false negative result can result from:
- presence of blood, bile, excess inorganic salts and other components in the feces sample;
- treatment with tetracycline, penicillin (aminopenicillins), macrolides (clarithromycin, azithromycin) and metronidazole.
Biological material
- Stool
Rules for preparing Kal PCR
MATERIAL COLLECTION RULES
- Feces cannot be sent for testing after an enema, insertion of suppositories, or intake of castor or vaseline oil.
- According to indications, the doctor prescribes a trial diet, which the patient adheres to for 4-5 days.
- Feces for testing should not contain foreign impurities, such...